rabbit polyclonal anti glast  (Novus Biologicals)

Journal: Cells

Article Title: Hypothermia Shifts Neurodegeneration Phenotype in Neonatal Human Hypoxic–Ischemic Encephalopathy but Not in Related Piglet Models: Possible Relationship to Toxic Conformer and Intrinsically Disordered Prion-like Protein Accumulation

doi: 10.3390/cells14080586

Figure Lengend Snippet: Antibodies Used for Cell Type and Synapse Identification in Combination with Antibodies to Toxic Conformer Proteins.

Article Snippet: GLAST , Astrocytes , Rabbit polyclonal , Proteintech, 20785-1-AP.

Techniques: Drug discovery, Ubiquitin Proteomics, Transduction

Journal: International journal of molecular sciences

Article Title: Neuroprotective Potential of L-Glutamate Transporters in Human Induced Pluripotent Stem Cell-Derived Neural Cells against Excitotoxicity.

doi: 10.3390/ijms241612605

Figure Lengend Snippet: Figure 3. Expression of EAAT1 and EAAT2 in hiPSC-derived neural cells. (A) The significant increase in the mRNA expression levels of EAAT1 (a1) and EAAT2 (a2) along with culture days was confirmed by qRT-PCR (n = 3). ** p < 0.01 vs. DIV 0 group, Tukey’s test following ANOVA. Data are expressed as the means ± standard deviations. Similar results were obtained in three independent experiments. (B) Representative immunoblot at 14 and 63 DIV (b1). The expression level of each marker was normalized to 14 DIV. The expression levels of EAAT1 (b2) and EAAT2 (b3) protein tended to increase with culture days. Similar results were obtained in four independent experiments. (C) Identification of cell types expressed EAAT1 and EAAT2 at 63 DIV. We used the following cell markers: GFAP+Nestin+ for radial glial cells, GFAP+S100β+ for astrocytes, and HuC/D+ or MAP2+ for neurons. (c1) EAAT1 was localized in radial glial cells (top) and astrocytes (bottom). (c2) EAAT2 was localized in radial glial cells (top), astrocytes (middle), and neurons (bottom). Scale bar, 100 µm. Similar results were obtained in three independent experiments.

Article Snippet: The membrane was incubated with mouse anti-HuC/D monoclonal antibody (1:100, A21271, Thermo Fisher Scientific), mouse anti-GFAP monoclonal antibody (1:5000, MAB3402, Millipore, Hessen, Germany), rabbit anti-S100β polyclonal antibody (1:5000, A5971, Sigma-Aldrich), rabbit anti-EAAT1 polyclonal antibody (1:1000, 5684, Cell Signaling, Beverly, MA, USA), guineapig anti-EAAT2 polyclonal antibody (1:5000, AB1783, Chemicom), or mouse anti β-actin monoclonal antibody (1:5000, ab8226, Abcam) overnight at 4 ◦C, followed by incubation with horseradish peroxidase-conjugated anti-mouse, anti-rabbit (1:20,000, Amersham Biosciences, Buckinghamshire, UK), or guineapig (1:50,000, Invitrogen, Waltham, MA, USA) antibodies.

Techniques: Expressing, Derivative Assay, Quantitative RT-PCR, Western Blot, Marker

Journal: iScience

Article Title: Robust induction of functional astrocytes using NGN2 expression in human pluripotent stem cells

doi: 10.1016/j.isci.2023.106995

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-SLC1A3 , Boster Biological Technology , Cat# PA2185 RRID:AB_2665510.

Techniques: Recombinant, Lysis, Software